WebbQubit 4 Qubit Flex Varioskan LUX; What is the lower concentration limit (dsDNA)? 0.005 ng/µL* 0.005 ng/µL* Depends on the Quant-iT assay kit, standard concentrations and curve fittings used** See details 0.005 ng/µL : What is the upper detection limit (dsDNA)? 4000 ng/µL* 4000 ng/µL* Depends on the Quant-iT assay kit and assay conditions WebbThe Qubit working solution was prepared by diluting the Qubit BR Reagent (200X) 1:200 in Qubit dsDNA BR Buffer. A 4-, 5-, 6- and 8-point serial log dilution series of standards ranging between 1000 ng/μL and 0.1 ng/μL was created from a 2.2 mg/mL stock solution of Herring Sperm DNA in TE buffer.
User Guide: Qubit dsDNA HS Assay Kits - Thermo Fisher Scientific
Webb用于 RNA 或 DNA 荧光定量的高性能常用仪器包括:用于单个样品微量荧光定量的 Invitrogen Qubit 4 荧光计、用于 8 孔样品微量荧光定量的 Invitrogen Qubit Flex 荧光计、基于滤光片进行96-384 孔微孔板荧光定量的 Thermo Scientific Fluoroskan 微孔板荧光分析仪,以及基于光栅进行96-1536孔微孔板进行荧光定量的 Thermo ... WebbDesigned specifically for Thermo’s Qubit® fluorometer Linear range: 2-1000 ng dsDNA Green fluorescence (Ex/Em: 500/530 nm) Replaces Qubit® dsDNA BR Assay Kit for cost savings A genomic DNA sample was quantified using both the Qubit® BR kit and the AccuGreen™ BR kit. A nearly identical result was obtained with each kit. AccuGreen™ … mover regla powerpoint
User Guide: Qubit dsDNA BR Assay Kits - Thermo Fisher Scientific
WebbdsDNA HS (High Sensitivity) Assay Kits make DNA quantitation easy and accurate. The kits include concentrated assay reagent, dilution buffer, and prediluted DNA standards. … Webb10 μL of Standard #2 is required for a single Qubit™ dsDNA HS assay standard curve. 1.3 Store the standards at 2–8°C along with other kit components for up to 6 months.. … WebbThe Qubit ™ dsDNA HS Assay is highly selective for double-stranded DNA (dsDNA) over RNA (Figure 2). Figure 2 DNA selectivity and sensitivity of the Qubit ™ dsDNA HS Assay. … mover reich montage