Nphs1-cre

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August undefined, 2024

WebThe NPHS1 gene mutated in NPHS1 children has recently been identified. The gene codes for nephrin, a cell-surface protein of podocytes. Two mutations, named Fin-major and Fin-minor, have been found in over 90% of the Finnish patients. WebNphs1‐Cre+Flt1flox/TK 33 (8/25) 24 (6‐10 weeks of age) Table S2. sFLT1 Binds to Ganglioside Extracted from Podocytes, as Measured by MRM Mass Spectrometry, Related to Figure 5

NPHS2 Gene - GeneCards PODO Protein PODO Antibody

WebView Tg(Nphs1-cre)#Seq allele: origin, molecular description, gene associations, and references. Web10 aug. 2024 · Crescentic glomerulonephritis encompasses a group of diverse disorders characterized by hyperplasia of parietal epithelial cells (PECs) in the space surrounding the glomerulus (so-called crescents) as the main histopathological lesion at kidney biopsy (1, 2).Crescents are frequently associated with a rapid decline in kidney function referred to … how to grow your roth ira

Nphs2-CreERT2

Webof Cre recombinase, they express IjBDN, which lacks its N-terminal of 54 amino acids including 2 phosphorylation sites at serines 32 and 36, thereby continuously inhibiting NF-jB activation as a super-repressor. In the present study, podocyte-speciﬁc IjBDN … WebThe Nphs1-Cre transgene mediates podocyte-specific recombination. (A)Podocyte-specific recombination of loxP-flanked regions mediated by the Nphs1-Cre transgene. Red triangles represent loxP sequences. (B)Specific Cre activity restricted to the glomeruli was confirmed in the kidneys of Rosa26R reporter mice carrying the Nphs1-Cre transgene. X-gal … Web神经研究专用 AAV. 由于中枢神经系统（Central nervous system，CNS）的复杂性，神经组织的有限再生能力，血脑屏障（Blood‒brain barrier，BBB）对药物输送的限制，采用传统药物和外科手术方法治疗神经系统疾病仍然很困难，目前科学家正在研究使用基因疗法治 … how to grow your restaurant business

Nphs1-cre

An Essential Role of the Universal Polarity Protein, aPKCλ, on the ...

Web20 jun. 2024 · Nephrin (Nphs1) is an adhesion protein that is expressed at the podocyte intercellular junction in the glomerulus. Nphs1 mutations in humans or deletion in animal genetic models results in a developmental failure of foot process formation. A number of … Web14 jan. 2009 · Glomerular visceral epithelial cells (podocytes) contain interdigitated processes that form specialized intercellular junctions, termed slit diaphragms, which provide a selective filtration barrier in the renal glomerulus.

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Web14 jan. 2009 · The nephrin–podocin complex is the main constituent of slit diaphragms. However, the molecular mechanisms regulating these proteins to maintain the slit diaphragms are still largely unknown. Here, we demonstrate that the PAR3–atypical … Web3.Cre-on，Flex，DIO，double floxed 这些技术名称经常在文献中被提及，其实都是一回事，就是使用 Cre-Loxp 系统，经过两次染色体重组在特定脏器中表达外源基因。前提是我们需要获得特定的 Cre 小鼠后才可以使用这个技术。 Cre-on 系统的技术原理如下：莱德盟 …

Web18 jan. 2024 · Nphs1-RiboTag and Nphs1-RiboTag-NEP25 mice without LMB2 showed a normal UACR of 0.085 mg/mg [95% confidence interval (CI) 0.076–0.096] and normal renal morphology. Glomeruli were isolated from Nphs1-RiboTag mice, homogenized, and … Web8 jan. 2024 · Putaala et al. have reported that Nphs1-Cre mice express Cre recombinase in podocytes at E13 or later, while Brunskill et al. demonstrated that Nphs2-Cre mice express Cre recombinase at E14.5 or ...

WebNephrin is encoded by the NPHS1 gene, and patients who harbour mutations in the NPHS1 gene develop heavy proteinuria before birth and rapidly progress to end stage renal failure, this is known as congenital nephrotic syndrome of the Finnish type (4, 5). ... Nphs1-Cre Tg mice) were described previously . WebNormal Function. The NPHS2 gene provides instructions for making a protein called podocin. Podocin is primarily found in the kidneys, which are organs that filter waste products from the blood and remove them in urine. Specifically, podocin is found in cells …

WebNphs1-Cre+Flt1flox/flox Reorganization of podocyte cytoskeleton, Kidney failure (35) Vegfr-1/Flt-1 germline TM domain knockout Flt-1TM-TK/TM-TK Reduced vascular development, death at E8.5 in some embryos (21) Vegfr-2/Flk-1 germline knockout with lacZ reporter Flk-1−/− (Flk-1lcz/lcz) Lack of vascular development, lethality at E8.5-9.5 (14 ...

WebCre 蛋白是一种重组酶，是causes recombination的缩写，于1981 年从 P1 噬菌体中被发现，属于 λInt 酶超基因家族。 Cre 重组酶基因编码区序列全长1029bp，编码 38kDa 蛋白质，Cre 重组酶是由 343 个氨基酸组成的单体蛋白。 how to grow your salon businessWebThe Nphs1-Cre transgene mediates podocyte-specific recombination. (A)Podocyte-specific recombination of loxP-flanked regions mediated by the Nphs1-Cre transgene. Red triangles represent loxP sequences. (B)Specific Cre activity restricted to the glomeruli was … john walcutt actorWeb图4、rAAV2-retro（逆向非跨突触标记）（Tervo et al., Neuron, 2016）二、可顺向跨单级突触的血清型. 2024年张砺教授团队研究报道AAV1能在高滴度条件下呈顺向跨突触传播，与Cre依赖的报告系统相结合，可高效和特异性地驱动Cre依赖的外源基因在突触后神经元 … john walcott actorWebTjp1 flox/flox, Tjp2 flox/flox and Nphs1-Cre mice, which drive Cre recombinase expression in podocytes under nephrin promoter, were described previously. The podocyte-specific ZO-2 knockout mice were obtained by crossing Tjp2 flox/flox mice and Nphs1-Cre : Tjp2 … how to grow your short hair outWeb3 sep. 2014 · Podocyte-specific deletion of Tjp1 results in glomerulosclerosis. (A) Kidney sections from Tjp1 flox/flox and Nphs1-Cre: Tjp1 flox/flox mice were examined with the antibody against Tjp1. how to grow your savings accountWebIntroduction: Few studies have addressed the genetic spectrum of NPHS1 variants in Chinese children with nephrotic syndrome. In this multicenter study, the clinical manifestations and features of NPHS1 variants in Chinese children with nephrotic syndrome were researched.Method: Genotypical and phenotypical data from 30 children affected … john walden home retail groupWebFlt1 allele, and the Nphs1-Cre transgene (Nphs1-Cre+Flt1ﬂox/TK). These mice can express full-length Flt1 as well as sFlt1 in all tissues except podocytes, in which the ﬂoxed allele is inacti-vated,leavingonlyFlt1TK.Outof33animalsthatwereexamined, the majority (n … how to grow your stock portfolio