Human regulatory t cell staining kit
Web16 dec. 2009 · In current study, human regulatory T cell staining kit no. 2 (eBioscience Inc., San Diego, CA, USA) was used for staining FOXP3 + CD4 + CD25 + Treg cells. The following monoclonal antibodies were included: anti-Foxp3-APC (Clone: PCH101), anti-CD4-FITC (Clone: RPA-T4), anti-CD25-PE (Clone: BC96).
Human regulatory t cell staining kit
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Web(Bottom) Staining of normal human peripheral blood cells with FITC anti-human CD4 (OKT4) (left) or APC anti-human CD25 (BC96) (right) followed by RBC lysis and … WebPRD013-201319 V1 PRODUCT INFORMATION SHEET Maxpar® Human Regulatory T Cell Phenotyping Panel Kit Catalog#: 201319 Package Size: 25 tests Storage: • Antibodies, Buffers, and Water: 4°C. Do not freeze. • Intercalator -Ir: 20°C. Contents: • Maxpar ® Cell Staining Buffer (500 mL) • Maxpar ® Nuclear Antigen Staining Buffer …
Web15 nov. 2006 · Activation-induced cell death (AICD) through CD95/CD95L has been described as such an apoptosis-inducing mechanism. 11 While naive T cells are CD95 – and resistant to apoptosis induction, activated T cells (CD45RO hi) up-regulate CD95 and become sensitive to apoptosis. Webcytometry. These kits allow for staining of surface CD4 along with intracellular factors such as IFN-γ, IL-4, IL-17A, and FoxP3. Depending on the kit, researchers can simultaneously look at Th1, Th2, and Th17 cells; Th1 and Th17 cells; or Th17 and regulatory T cells in mouse or human model systems. Essential tools for research in the area of T ...
Web17 mei 2024 · 天然CD4+ CD25+调节性T细胞 ( regulatory Tcell,Treg )为一类具有免疫抑制作用的T细胞,约占外周CD4+ T细胞的5~10%。 该类细胞以'主动'的方式参与免疫应 … Webregulatory T cells were isolated from human PBMCs. The cells were fluorescently stained as indicated and analyzed by flow cytometry using the MACSQuant® Analyzer. Gating …
WebHuman Regulatory T Cell Staining Kit; eBioscience: Contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and …
WebThis kit simplifies the TCR Vβ repertoire analysis by reducing the number of tubes to analyze (8 instead of 24) and consequently the time required for obtaining the results. Moreover, this kit makes it possible to study the repertoire on T cell subsets, using additional T-cell markers, such as CD3, CD4, and CD8, conjugated to a third fluorophore. right method electricWeb1 dec. 2013 · Human regulatory T cell staining kit (eBioscience), which including FITC-labeled CD4 antibody, APC-labeled CD25 antibody and PE-labeled Foxp3 antibody, were used to detect the frequency of Treg (CD25 + Foxp3 + /CD4 + cells) in the PBMCs. right meyer\u0027s loop lesionWebAll steps described below for magnetic labeling and depletion of non-CD4 + and CD127 high cells, as well as labeling and selection of CD25 + cells, are performed using the CD4 + … right method right result investigationWebThe Rat Regulatory T Cell Multi-Color Flow Cytometry Kit includes three fluorochrome-conjugated primary antibodies, isotype controls, and buffers to fix, permeabilize, and wash cells. CD25-PE (goat IgG) CD4-FITC (Clone OX-38; mouse IgG 2A) FoxP3-APC (Goat IgG) Goat IgG-APC Isotype Control right mid abdomenWeb30 jul. 2024 · Regulatory T cells (Tregs), are immune cells that are responsible for the maintenance of immunological tolerance to self-antigens, as well as for the suppression of excessive immune responses, which would otherwise have deleterious consequences. right mfWebThe BD™ Human Regulatory T Cell Sorting Kit provides reagents that allow investigators to stain viable human peripheral blood mononuclear cells (PBMC) or an enriched CD4+ … right mfg concreteWebHuman Regulatory T Cell Staining Kit 人调节性T细胞染色试剂盒; ¥ – ¥; 说明书; 在售SKU:70-KTR101-100, 70-KTR101-25,人,human,Treg,Tregs,调节性T细胞,调节,T细胞,调 … right mid arm pain