WebFeb 7, 2024 · LGC BioSearch Technologies: Cat#SMF-WA1-60: Stellaris RNA FISH Wash Buffer B: LGC BioSearch Technologies: Cat#SMF-WB1-20: ProLong Antifade Mountant with DAPI: Invitrogen: Cat#P36935: TRIzol Reagent: Invitrogen: Cat#15596026: ECL western blotting substrate: Thermo Scientific: Cat#32106: DreamTaq DNA polymerases: Web2 mL Stellaris RNA FISH Wash Buffer A (LGC Biosearch Technologies Cat# SMF-WA1-60) Add 7 mL Nuclease-free water Add 1 mL Deionized Formamide Mix well by vortexing gently Wash Buffer B: Add Nuclease-free water to Wash Buffer B bottle upon first use. Add 88 mL of Nuclease-free water to bottle (LGC Biosearch Technologies Cat# SMF-WB1 …
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WebFluorescence In Situ Hybridization (FISH) Stellaris RNA FISH Hybridization & Wash Buffers SMF-WA1-60-BS Stellaris® RNA FISH Wash Buffer A Product Description Stellaris® … WebApr 8, 2024 · #SMF-WA1-60; Biosearch technologies) for 5 min. Then the miR-200c-3p, Dll4 mRNA and Notch1 mRNA probes were mixed with the hybridization buffer (cat. # SMF-HB1-10; Biosearch technologies) according to the manufacturer’s instructions, covered in foil and placed in the cell incubator at 37°C for 5 h, all subsequent steps were performed … citibank harassing phone calls
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WebThe permeabilized samples were washed once with wash buffer A (10% Formamide (Ambion, 9342), 20% Stellaris RNA FISH Wash Buffer A (Biosearch Technologies Cat# SMF-WA1-60) in nuclease-free water ... Web2 mL Stellaris RNA FISH Wash Buffer A (Biosearch Technologies Cat# SMF-WA1-60) Add 7 mL Nuclease-free water Add 1 mL Deionized Formamide Mix well by vortexing gently. Wash Buffer B: Add Nuclease-free water to Wash Buffer B bottle upon first use. Add 88 mL of Nuclease-free water to bottle (Biosearch Technologies Cat# SMF-WB1-20) before use. WebJul 19, 2024 · Slides were washed with Stellaris wash A buffer (LGC Biosearch Technologies, Cat. No. SMF-WA1-60) and incubated with Stellaris fluorescent mRNA probes in the dark at 37°C overnight (LGC Biosearch Technology, SOD1 [Custom assay], GFP [Cat. No. VSMF-1014-5], GAPDH [Cat. No. SMF-3002-1], beta-actin) in … diaper allergy face